Science diary

In last post we discussed about sterilization and its physical methods. In this post we will discuss about sterilization and its chemical methods in the most precise way. By this far we know that sterilization can be defined as the process of killing all microorganisms (including spores) on or in a material or object.  We also know that heating is the most common and reliable method for sterilization.  Here are some chemical methods of sterilization: 1) Gases: Types used in sterilization: a) Ethylene oxide b) Formaldehyde gas: This is widely employed for fumigation of OT and other rooms. c) Betapropiolactone:  -having rapid bactericidal activity but carcinogenic. - capable of killing all microorganisms and is very active against viruses. 2) Surface active agents:  Substances that alter the energy relationship at interfaces, producing a reduction Of surface or interfacial tension is called surface active agents. They are widely used as wetting agents, emulsifiers and detergents. These a

STERILIZATION is a process of killing all micro organisms (including spores) on or in a material or object. Heating is the most common method used for sterilization. Factors that determine the type of sterilization include: 1) Heat,  2) Temperature. Eg: pasteurization of milk holding period is 63° for 30 minutes or 72°c for 15-20 min. For killing all non sporing pathogens. Eg: mycobacteria, brucellae, salmonella.  3) Stages growth of organisms. 4) Nature of medium in which the organism       is suspended (air, gas, liquid) 5) Number of organisms present. Thus making a substance free from all the microorganisms both in vegetative and sporing states. It is the most important procedure and has major share in success of surgical management.  Sterilization and disinfection can be achieved by using heat filtration, chemical or radiation etc.. Some are discussed below. Methods of sterilization are:  1) Physical Methods  2) Chemical Methods  Some general used laboratory method for sterilizatio